Journal: Journal of Agricultural and Food Chemistry

Article Title: In Vitro and In Vivo Insights into a Broccoli Byproduct as a Healthy Ingredient for the Management of Alzheimer’s Disease and Aging through Redox Biology

doi: 10.1021/acs.jafc.3c05609

Figure Lengend Snippet: Effect of broccoli byproduct extract (BRO) 100 μg/mL on oxidative stress markers on aging (young worms = 5 days old; old worms = 12 days old). (A) Intracellular reactive oxygen species (ROS) content measured by the DCFDA technique. (B) Representative dot plots panels of intracellular ROS content vs TOF (time-of-flight; worm size) of yellow fluorescence intensity extracted from flow cytometer software. (C) Mitochondrial ROS content measured by Mitotracker staining. (D) Representative dot plots panels of mitochondrial ROS content vs TOF of red fluorescence intensity extracted from flow cytometer software. (E) Lipofuscin content. (F) Representative images of lipofuscin for each group (10× magnification) obtained by using an epi-fluorescence microscope with a DAPI (blue) filter. Lowercase letters, when different, represent statistically significant differences ( p < 0.05). Results are expressed as mean ± SEM. CTL = control group.

Article Snippet: Thioflavin T-stained worms were visualized using a Nikon epi-fluorescence microscope (Eclipse Ni, Nikon, Tokyo, Japan) at 40× magnification.

Techniques: Fluorescence, Flow Cytometry, Software, Staining, Microscopy